Remember with shots like this to not go for the 2.8 aperture, but rather a higher one to carry the clearity through the focus plane. Being as close as you are to the eye, I would set my camera up on manual at Shutter speed 250 (minimum) Aperture f.11 (or close to it)and ISO 200-400.
Give it a whirl. :)
Remember that the above recipe will get you around the area of a really clear shot, but most likely you will need additional experimenting to get "the perfect" results.

Thank you very much,I'll give it a shot!I'm always ready for suggestions...
Anything that may help.